The Dispersion of Diaspores of Protium icicariba (Burseraceae) - a Networked or Multifactorial System?

The adaptive radiation of the angiosperms was strongly affected by fruit and seed dispersal since the establishment of the seedlings is a fundamental process for the recruitment of juveniles to the populations. Among the species of Burseraceae, seeds with fleshy attachments and high caloric value suggest mammaliochory as an ancestral dispersal way. In Protium icicariba, at the same time as there is a visual pattern typical of ornithochory, with a report of effective demonstration, the diaspores present the highest levels of essential oils of the whole plant, suggesting other dispersion processes by olfactory guided vectors. This work aims to monitor the diasporic dispersal process in P. icicariba in situ, aiming to identify dispersers and to investigate the role of the essential oil in the dispersion of diaspores of this plant species. The natural dispersion was monitored in situ, in weekly campaigns throughout eight months, using visual and photographic records, in daily shifts of six hours, distributed along the dawn, morning, afternoon, dusk, and night. We used both direct observation and continuous picture capturing along 43 days with photographic traps. Mature diaspores removed from pseudocapsules were pooled to determine potential dispersers. Artificial models of the diaspores, in white and green colors, were also used to test hypotheses on the role of scent in the dispersion, added 1%, weight/weight, of the essential oil extracted from the mature diaspores, which chemical composition determined by gas chromatography coupled to mass spectrometry. Besides, the analysis of stomach contents of lizards collected in adjacent area was also performed. In daytime and nighttime monitoring in nature, no vertebrates were recorded dispersing diaspores. The most common was the primary wind-facilitated autochory of diaspores to the substrate, near the plant matrices. Secondarily, workers of the ant species Atta robusta can remove the pseudoarils or move the pyrenes to the anthills. The lizard species Tropidurus torquatus ingests pyrenes with the pseudoarils, and the sclerified pericarp of the pyrene is potentially resistant to chemical action of the digestive juices. Ants and lizards have also accessed the caves with natural diaspores. Concerning the artificial diaspore models, ants accessed, indistinctly, white and the green models that contained essential oils. The lizards accessed the white models, with or without essential oils, and showed insignificant access to green ones, with or without essential oil. The ingestion of pyrenes by lizards was also confirmed through analysis of stomach contents. The aggregate spatial pattern of P. icicariba at the study site, associated with clumps, may be derived from germination in the substrate near the matrices, or in the anthills or after diaspora defecation and / or regurgitation of the lizard, which is a species strongly associated with clumps of this vegetation. As the access to the diaspores by ants and lizards depends on the primary autochory, and no impediments to the germination near to the matrix plant were found, the dispersion is compatible with a multifactorial characteristic of the diplochory.

Biochemical and cellularchanges in Oreochromis niloticus related to the water pollution of a degraded river / Alterações bioquímicas e celulares em Oreochromis niloticus relacionadas com a poluição da água de um rio degradado

The effects of polluted water at three sites in the Marinho River, Brazil, on Oreochromis niloticus (Nile tilápia) were investigated using histological, hematological and biochemical approaches. Fish exposed to the impacted water demonstrated that histological changes in gills were accompanied by nuclear and micronuclei abnormalities in cells. The activity of liver and plasma biomarkers (alkaline phosphatase (ALP), acid phosphatase (ACP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver glutathione S-transferase (GST)) showed an expressive change due to the. The results were also correlated with the highest levels of Cu+2, Zn+2 and Mn+2 in the water. The data of this study evidenced the importance of using a set of biomarkers to quantify pollution in lentic ecosystems. Additionally, histological analyses of gills and erythrocytes have proven to be an important instrument for signaling the impact of pollutants in rivers.

Os efeitos da poluição da água de três locais do Rio Marinho, Brasil, em Oreochromis niloticus foram investigados usando técnicas histológicas, hematológicas e bioquímicas. Peixes expostos à água impactada demonstraram que alterações histológicas nas brânquias foram acompanhadas de anomalias nucleares e micronúcleo nas células. A determinação da atividade de biomarcadores em fígado e plasma de tilápia (fosfatase alcalina (ALP), fosfatase ácida (ACP), alanina aminotransferase (ALT), aspartato aminotransferase (AST) e glutationa S-transferase (GST)) mostrou uma substancial alteração em função da poluição. Os resultados são correlacionados com os níveis mais elevados de Cu+2, Zn+2 e Mn+2 na água. Os dados deste estudo demonstram a importância de utilizar um conjunto de biomarcadores para quantificar a poluição em ecossistemas lênticos. Adicionalmente, as análises histológicas das brânquias e de eritrocitos têm provado ser importante instrumento para sinalizar a impactação de poluentes ao longo de rios.

Alongamento muscular na dor lombar crônica inespecífica: uma estratégia do método GDS / Stretching in nonspecific chronic low back pain: a strategy of the GDS method

O objetivo deste estudo foi verificar a eficácia do alongamento muscular, usando uma sequência proposta pelo Método Godelieve Denys-Struyf (GDS) na redução da dor, na incapacidade funcional, no aumento da flexibilidade global e na capacidade de contração do músculo transverso do abdome (TrA), em indivíduos com dor lombar crônica inespecífica. Participaram 55 pacientes, de 18 a 60 anos, divididos em dois grupos: Grupo Alongamento (n=30) submetido a exercícios de alongamento, duas vezes por semana, e Grupo Controle (n=25) que não realizou tratamento. A dor foi avaliada pela escala visual analógica; a incapacidade funcional, pelo Índice de Oswestry; a flexibilidade global, pelo terceiro dedo ao solo; e a capacidade de contração do TrA, pela unidade de biofeedback pressórico. Foram realizadas três avaliações, inicial, após 8 e 16 semanas da inicial. Foi considerado nível de significância de α<0,05. Os resultados mostram que o Grupo Alongamento apresentou diminuição na dor, incapacidade funcional e aumentou a flexibilidade global (p<0,001) após 8 e 16 semanas (p<0,05), porém não melhorou a capacidade de contração do TrA (p=0,13). A sequência de alongamentos usada no método GDS mostra-se eficaz na redução da dor, incapacidade funcional e melhora da flexibilidade global em pacientes com dor lombar crônica inespecífica.

The purpose of this study was to assess the effectiveness of stretching exercises of the Muscle and Joint Chains Godelieve Denys-Struyf (GDS) method in pain reduction, functional disability, increase global flexibility, and ability of the transversus abdominis muscle (TrA) contraction in individuals with nonspecific chronic low back pain. Fifty-five patients, from 18 to 60 years-old, participated in this study divided into two groups: the Stretching Group (n=30) underwent stretching exercises twice a week; the Control Group (n=25) was subjected only to evaluation. Pain was assessed by a visual analogue scale, functional disability by the Oswestry Questionnaire, global flexibility by third finger to the ground test and the ability of TrA contraction by a pressure biofeedback unit. Three evaluations were performed, starting after 8 and 16 weeks from the beginning. Significance level was α<0.05. Results show that the Stretching Group presented a decrease in pain, functional disability, and increased global flexibility (p<0.001) after 8 and 16 weeks (p<0.05). No improvement in the ability of TrA muscle contraction was demonstrated (p=0.13). The sequence of stretching exercises used in the GDS method is effective in reducing pain, functional disability, and improving global flexibility in patients with nonspecific chronic low back pain.